Anti-NDUFB10 Antibody (CAB3982)
- SKU:
- CAB3982
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-NDUFB10 Antibody |
| Antibody SKU: | CAB3982 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IP |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-172 of human NDUFB10 (NP_004539.1). |
| Application: | WB IHC IP |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:100 IP 1:50 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HepG2, HeLa, MCF-7, Jurkat, Mouse brain, Mouse kidney, Mouse heart |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-172 of human NDUFB10 (NP_004539.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MPDS WDKD VYPE PPRR TPVQ PNPI VYMM KAFD LIVD RPVT LVRE FIER QHAK NRYY YYHR QYRR VPDI TECK EEDI MCMY EAEM QWKR DYKV DQEI INIM QDRL KACQ QREG QNYQ QNCI KEVE QFTQ VAKA YQDR YQDL GAYS SARK CLAK QRQR MLQE RKAA KEAA AATS |
| Gene ID: | 4716 |
| Uniprot: | O96000 |
| Cellular Location: | Matrix side, Mitochondrion inner membrane, Peripheral membrane protein |
| Calculated MW: | 20kDa |
| Observed MW: | 21kDa |
| Synonyms: | NDUFB10, PDSW |
| Background: |
| UniProt Protein Function: | NDUFB10: Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Belongs to the complex I NDUFB10 subunit family. |
| UniProt Protein Details: | Protein type:Oxidoreductase; Mitochondrial; EC 1.6.5.3; Energy Metabolism - oxidative phosphorylation; EC 1.6.99.3 Chromosomal Location of Human Ortholog: 16p13.3 Cellular Component: mitochondrial inner membrane; mitochondrial respiratory chain complex I Molecular Function:protein binding; NADH dehydrogenase (ubiquinone) activity Biological Process: cellular metabolic process; mitochondrial electron transport, NADH to ubiquinone |
| UniProt Code: | O96000 |
| NCBI GenInfo Identifier: | 6093491 |
| NCBI Gene ID: | 4716 |
| NCBI Accession: | O96000.3 |
| UniProt Secondary Accession: | O96000,Q96II6, |
| UniProt Related Accession: | O96000 |
| Molecular Weight: | 172 |
| NCBI Full Name: | NADH dehydrogenase |
| NCBI Synonym Full Names: | NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 10, 22kDa |
| NCBI Official Symbol: | NDUFB10 |
| NCBI Official Synonym Symbols: | PDSW |
| NCBI Protein Information: | NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10; NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10; CI-PDSW; complex I-PDSW; complex I PDSW subunit; NADH-ubiquinone oxidoreductase PDSW subunit; NADH ubiquinone oxidoreductase PDSW subunit (RH 16p13.3) |
| UniProt Protein Name: | NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 |
| UniProt Synonym Protein Names: | Complex I-PDSW; CI-PDSW; NADH-ubiquinone oxidoreductase PDSW subunit |
| UniProt Gene Name: | NDUFB10 |
| UniProt Entry Name: | NDUBA_HUMAN |
 | Western blot analysis of extracts of various cell lines, using NDUFB10 antibody (CAB3982) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
 | Immunohistochemistry of paraffin-embedded mouse stomach using NDUFB10 antibody (CAB3982) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded rat kidney using NDUFB10 antibody (CAB3982) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human stomach using NDUFB10 antibody (CAB3982) at dilution of 1:100 (40x lens). |
 | Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug NDUFB10 antibody (CAB3982). Western blot was performed from the immunoprecipitate using NDUFB10 antibody (CAB3982) at a dilition of 1:1000. |
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