Anti-GRK4 항체 (CAB10370)

Antibody Name:	Anti-GRK4 Antibody
Antibody SKU:	CAB10370
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 359-578 of human GRK4 (NP_892027.2).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse, Rat
Positive Samples:	U-87MG, BT-474, HeLa, Mouse brain, Mouse lung, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 359-578 of human GRK4 (NP_892027.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	NNEK YTFS PDWW GLGC LIYE MIQG HSPF KKYK EKVK WEEV DQRI KNDT EEYS EKFS EDAK SICR MLLT KNPS KRLG CRGE GAAG VKQH PVFK DINF RRLE ANML EPPF CPDP HAVY CKDV LDIE QFSV VKGI YLDT ADED FYAR FATG CVSI PWQN EMIE SGCF KDIN KSES EEAL PLDL DKNI HTPV SRPN RGFF YRLF RRGG CLTM VPSE KEVE PKQC
Gene ID:	2868
Uniprot:	P32298
Cellular Location:	Cytoplasm, cell cortex
Calculated MW:	57kDa/61kDa/63kDa/66kDa
Observed MW:	72kDa

Synonyms:

GRK4, GPRK2L, GPRK4, GRK4a, IT11

Background:

This gene encodes a member of the guanine nucleotide-binding protein (G protein)-coupled receptor kinase subfamily of the Ser/Thr protein kinase family. The protein phosphorylates the activated forms of G protein-coupled receptors thus initiating its deactivation. This gene has been linked to both genetic and acquired hypertension. Several transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	GRK4: Specifically phosphorylates the activated forms of G protein-coupled receptors. GRK4-alpha can phosphorylate rhodopsin and its activity is inhibited by calmodulin; the other three isoforms do not phosphorylate rhodopsin and do not interact with calmodulin. GRK4-alpha and GRK4-gamma phosphorylate DRD3. Phosphorylates ADRB2. Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily. 4 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:AGC group; EC 2.7.11.16; GRK family; GRK subfamily; Kinase, protein; Protein kinase, AGC; Protein kinase, Ser/Thr (non-receptor) Chromosomal Location of Human Ortholog: 4p16.3 Molecular Function:rhodopsin kinase activity Biological Process: receptor internalization; regulation of G-protein coupled receptor protein signaling pathway; regulation of rhodopsin mediated signaling
NCBI Summary:	This gene encodes a member of the guanine nucleotide-binding protein (G protein)-coupled receptor kinase subfamily of the Ser/Thr protein kinase family. The protein phosphorylates the activated forms of G protein-coupled receptors thus initiating its deactivation. This gene has been linked to both genetic and acquired hypertension. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2013]
UniProt Code:	P32298
NCBI GenInfo Identifier:	143811400
NCBI Gene ID:	2868
NCBI Accession:	P32298.3
UniProt Secondary Accession:	P32298,O00641, O00642, Q13293, Q13294, Q13295, Q14453 Q14725, Q15313, Q15314, Q15315, Q15316,
UniProt Related Accession:	P32298
Molecular Weight:	67kDa
NCBI Full Name:	G protein-coupled receptor kinase 4
NCBI Synonym Full Names:	G protein-coupled receptor kinase 4
NCBI Official Symbol:	GRK4
NCBI Official Synonym Symbols:	IT11; GPRK4; GRK4a; GPRK2L
NCBI Protein Information:	G protein-coupled receptor kinase 4
UniProt Protein Name:	G protein-coupled receptor kinase 4
UniProt Synonym Protein Names:	G protein-coupled receptor kinase GRK4; ITI1
Protein Family:	G protein-coupled receptor kinase
UniProt Gene Name:	GRK4

Western blot analysis of extracts of various cell lines, using GRK4 antibody (CAB10370) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.

Antibodies

GRK4 Antibody (PACO03958)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)