Anti-DTL Antibody (CAB12150)
- SKU:
- CAB12150
- Product type:
- Antibody
- Reactivity:
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-DTL Antibody |
| Antibody SKU: | CAB12150 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 541-730 of human DTL (NP_057532.3). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Mouse, Rat |
| Positive Samples: | Mouse testis, Mouse liver, Rat brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 541-730 of human DTL (NP_057532.3). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | AEAC SESR NRVK RRLD SSCL ESVK QKCV KSCN CVTE LDGQ VENL HLDL CCLA GNQE DLSK DSLG PTKS SKIE GAGT SISE PPSP ISPY ASES CGTL PLPL RPCG EGSE MVGK ENSS PENK NWLL AMAA KRKA ENPS PRSP SSQT PNSR RQSG KTLP SPVT ITPS SMRK ICTY FHRK SQED FCGP EHST EL |
| Gene ID: | 51514 |
| Uniprot: | Q9NZJ0 |
| Cellular Location: | Chromosome, Cytoplasm, Nucleoplasmic side, Nucleus, Nucleus membrane, Peripheral membrane protein, centrosome, cytoskeleton, microtubule organizing center |
| Calculated MW: | 23kDa/79kDa |
| Observed MW: | 90kDa |
| Synonyms: | DTL, CDT2, DCAF2, L2DTL, RAMP |
| Background: |
| UniProt Protein Function: | RAMP: Substrate-specific adapter of a DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex required for cell cycle control, DNA damage response and translesion DNA synthesis. The DCX(DTL) complex, also named CRL4(CDT2) complex, mediates the polyubiquitination and subsequent degradation of CDT1 and CDKN1A/p21(CIP1). CDT1 degradation in response to DNA damage is necessary to ensure proper cell cycle regulation of DNA replication. CDKN1A/p21(CIP1) degradation during S phase or following UV irradiation is essential to control replication licensing. Most substrates require their interaction with PCNA for their polyubiquitination: substrates interact with PCNA via their PIP-box, and those containing the 'K+4' motif in the PIP box, recruit the DCX(DTL) complex, leading to their degradation. In undamaged proliferating cells, the DCX(DTL) complex also promotes the 'Lys-164' monoubiquitination of PCNA, thereby being involved in PCNA-dependent translesion DNA synthesis. Belongs to the WD repeat cdt2 family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Adaptor/scaffold; Ubiquitin ligase Chromosomal Location of Human Ortholog: 1q32.3 Cellular Component: centrosome; cytosol; nucleolus; nucleoplasm; nucleus Molecular Function:protein binding; ubiquitin-protein ligase activity Biological Process: bypass DNA synthesis; DNA damage response, detection of DNA damage; G2/M transition DNA damage checkpoint; protein monoubiquitination; protein polyubiquitination; regulation of cell cycle; response to DNA damage stimulus; response to UV; ubiquitin-dependent protein catabolic process |
| UniProt Code: | Q9NZJ0 |
| NCBI GenInfo Identifier: | 302393825 |
| NCBI Gene ID: | 51514 |
| NCBI Accession: | Q9NZJ0.3 |
| UniProt Secondary Accession: | Q9NZJ0,Q5VT77, Q96SN0, Q9NW03, Q9NW34, Q9NWM5, A8K8H8 D3DT98, |
| UniProt Related Accession: | Q9NZJ0 |
| Molecular Weight: | 79kDa |
| NCBI Full Name: | Denticleless protein homolog |
| NCBI Synonym Full Names: | denticleless E3 ubiquitin protein ligase homolog |
| NCBI Official Symbol: | DTL |
| NCBI Official Synonym Symbols: | CDT2; RAMP; DCAF2; L2DTL |
| NCBI Protein Information: | denticleless protein homolog |
| UniProt Protein Name: | Denticleless protein homolog |
| UniProt Synonym Protein Names: | DDB1- and CUL4-associated factor 2; Lethal(2) denticleless protein homolog; Retinoic acid-regulated nuclear matrix-associated protein |
| Protein Family: | Denticleless protein |
| UniProt Gene Name: | DTL |
 | Western blot analysis of extracts of various cell lines, using DTL antibody (CAB12150) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
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