Anti-CRYGS 항체 (CAB7888)

Antibody Name:	Anti-CRYGS Antibody
Antibody SKU:	CAB7888
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-178 of human CRYGS (NP_060011.1).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse, Rat
Positive Samples:	Mouse eye, Rat eye

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-178 of human CRYGS (NP_060011.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MSKT GTKI TFYE DKNF QGRR YDCD CDCA DFHT YLSR CNSI KVEG GTWA VYER PNFA GYMY ILPQ GEYP EYQR WMGL NDRL SSCR AVHL PSGG QYKI QIFE KGDF SGQM YETT EDCP SIME QFHM REIH SCKV LEGV WIFY ELPN YRGR QYLL DKKE YRKP IDWG AASP AVQS FRRI VE
Gene ID:	1427
Uniprot:	P22914
Cellular Location:
Calculated MW:	21kDa
Observed MW:	21kDa

Synonyms:

CRYGS, CRYG8, CTRCT20

Background:

Crystallins are separated into two classes: taxon-specific, or enzyme, and ubiquitous. The latter class constitutes the major proteins of vertebrate eye lens and maintains the transparency and refractive index of the lens. Since lens central fiber cells lose their nuclei during development, these crystallins are made and then retained throughout life, making them extremely stable proteins. Mammalian lens crystallins are divided into alpha, beta, and gamma families; beta and gamma crystallins are also considered as a superfamily. Alpha and beta families are further divided into acidic and basic groups. Seven protein regions exist in crystallins: four homologous motifs, a connecting peptide, and N- and C-terminal extensions. Gamma-crystallins are a homogeneous group of highly symmetrical, monomeric proteins typically lacking connecting peptides and terminal extensions. They are differentially regulated after early development. This gene encodes a protein initially considered to be a beta-crystallin but the encoded protein is monomeric and has greater sequence similarity to other gamma-crystallins. This gene encodes the most significant gamma-crystallin in adult eye lens tissue. Whether due to aging or mutations in specific genes, gamma-crystallins have been involved in cataract formation.

UniProt Protein Function:	CRYGS: Crystallins are the dominant structural components of the vertebrate eye lens. Belongs to the beta/gamma-crystallin family.
UniProt Protein Details:	Chromosomal Location of Human Ortholog: 3q27.3 Disease: Cataract 20, Multiple Types
NCBI Summary:	Crystallins are separated into two classes: taxon-specific, or enzyme, and ubiquitous. The latter class constitutes the major proteins of vertebrate eye lens and maintains the transparency and refractive index of the lens. Since lens central fiber cells lose their nuclei during development, these crystallins are made and then retained throughout life, making them extremely stable proteins. Mammalian lens crystallins are divided into alpha, beta, and gamma families; beta and gamma crystallins are also considered as a superfamily. Alpha and beta families are further divided into acidic and basic groups. Seven protein regions exist in crystallins: four homologous motifs, a connecting peptide, and N- and C-terminal extensions. Gamma-crystallins are a homogeneous group of highly symmetrical, monomeric proteins typically lacking connecting peptides and terminal extensions. They are differentially regulated after early development. This gene encodes a protein initially considered to be a beta-crystallin but the encoded protein is monomeric and has greater sequence similarity to other gamma-crystallins. This gene encodes the most significant gamma-crystallin in adult eye lens tissue. Whether due to aging or mutations in specific genes, gamma-crystallins have been involved in cataract formation. [provided by RefSeq, Jul 2008]
UniProt Code:	P22914
NCBI GenInfo Identifier:	4033688
NCBI Gene ID:	1427
NCBI Accession:	P22914.4
UniProt Secondary Accession:	P22914,B2RAF8,
UniProt Related Accession:	P22914
Molecular Weight:	21,007 Da
NCBI Full Name:	Beta-crystallin S
NCBI Synonym Full Names:	crystallin gamma S
NCBI Official Symbol:	CRYGS
NCBI Official Synonym Symbols:	CRYG8; CTRCT20
NCBI Protein Information:	beta-crystallin S
UniProt Protein Name:	Beta-crystallin S
UniProt Synonym Protein Names:	Gamma-S-crystallin; Gamma-crystallin S
Protein Family:	Beta-crystallin
UniProt Gene Name:	CRYGS
UniProt Entry Name:	CRBS_HUMAN

Western blot analysis of extracts of various cell lines, using CRYGS antibody (CAB7888) at 1:4000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)