Anti-AP1M2 항체 (CAB8331)

Antibody Name:	Anti-AP1M2 Antibody
Antibody SKU:	CAB8331
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 164-423 of human AP1M2 (NP_005489.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:200
Reactivity:	Human, Mouse
Positive Samples:	22Rv1, BT-474, HepG2, Mouse kidney, Mouse lung, Mouse small intestine

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 164-423 of human AP1M2 (NP_005489.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	IKYK KNEV FIDV IESV NLLV NANG SVLL SEIV GTIK LKVF LSGM PELR LGLN DRVL FELT GRSK NKSV ELED VKFH QCVR LSRF DNDR TISF IPPD GDFE LMSY RLST QVKP LIWI ESVI EKFS HSRV EIMV KAKG QFKK QSVA NGVE ISVP VPSD ADSP RFKT SVGS AKYV PERN VVIW SIKS FPGG KEYL MRAH FGLP SVEK EEVE GRPP IGVK FEIP YFTV SGIQ VRYM KIIE KSGY QALP WVRY ITQS GDYQ LRTS
Gene ID:	10053
Uniprot:	Q9Y6Q5
Cellular Location:	Cytoplasmic side, Cytoplasmic vesicle, Golgi apparatus, Peripheral membrane protein, clathrin-coated vesicle membrane
Calculated MW:	48kDa
Observed MW:	48kDa

Synonyms:	AP1M2, AP1-mu2, HSMU1B, MU-1B, MU1B, mu2
Background:	This gene encodes a subunit of the heterotetrameric adaptor-related protein comlex 1 (AP-1), which belongs to the adaptor complexes medium subunits family. This protein is capable of interacting with tyrosine-based sorting signals. Alternative splicing results in multiple transcript variants.

UniProt Protein Function:	AP1M2: Subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the trans-Golgi network (TGN) and endosomes. The AP complexes mediate the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules. Belongs to the adaptor complexes medium subunit family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Chromosomal Location of Human Ortholog: 19p13.2 Cellular Component: Golgi membrane; cytoplasmic vesicle membrane; clathrin adaptor complex; intracellular membrane-bound organelle; lysosomal membrane; clathrin coated vesicle membrane; trans-Golgi network membrane; cytosol Biological Process: viral reproduction; vesicle targeting; antigen processing and presentation of exogenous peptide antigen via MHC class II; post-Golgi vesicle-mediated transport; regulation of defense response to virus by virus; protein targeting
NCBI Summary:	This gene encodes a subunit of the heterotetrameric adaptor-related protein comlex 1 (AP-1), which belongs to the adaptor complexes medium subunits family. This protein is capable of interacting with tyrosine-based sorting signals. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]
UniProt Code:	Q9Y6Q5
NCBI GenInfo Identifier:	13123953
NCBI Gene ID:	10053
NCBI Accession:	Q9Y6Q5.4
UniProt Secondary Accession:	Q9Y6Q5,Q9BSI8, B2RDV5,
UniProt Related Accession:	Q9Y6Q5
Molecular Weight:	48,210 Da
NCBI Full Name:	AP-1 complex subunit mu-2
NCBI Synonym Full Names:	adaptor-related protein complex 1, mu 2 subunit
NCBI Official Symbol:	AP1M2
NCBI Official Synonym Symbols:	mu2; MU1B; MU-1B; HSMU1B; AP1-mu2
NCBI Protein Information:	AP-1 complex subunit mu-2; mu-adaptin 2; mu1B-adaptin; HA1 47 kDa subunit 2; AP-mu chain family member mu1B; golgi adaptor AP-1 47 kDa protein; clathrin coat assembly protein AP47 2; clathrin coat associated protein AP47 2; adaptor protein complex AP-1 mu-2 subunit; golgi adaptor HA1/AP1 adaptin mu-2 subunit; clathrin-associated adaptor medium chain mu2; clathrin assembly protein complex 1 mu-2 medium chain 2
UniProt Protein Name:	AP-1 complex subunit mu-2
UniProt Synonym Protein Names:	AP-mu chain family member mu1B; Adaptor protein complex AP-1 subunit mu-2; Adaptor-related protein complex 1 subunit mu-2; Clathrin assembly protein complex 1 mu-2 medium chain 2; Golgi adaptor HA1/AP1 adaptin mu-2 subunit; Mu-adaptin 2; Mu1B-adaptin
Protein Family:	AP-1 complex
UniProt Gene Name:	AP1M2
UniProt Entry Name:	AP1M2_HUMAN

	Western blot analysis of extracts of various cell lines, using AP1M2 antibody (CAB8331) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 1s.
	Immunohistochemistry of paraffin-embedded mouse stomach using AP1M2 antibody (CAB8331) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human liver damage using AP1M2 antibody (CAB8331) at dilution of 1:100 (40x lens).
	Confocal immunofluorescence analysis of U2OS cells using Anti-AP1M2 Polyclonal Antibody (CAB8331) at dilution of 1:200. Blue: DAPI for nuclear staining.

Antibodies

AP1M2 Antibody (PACO07765)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)