Anti-ADD1 Antibody (CAB1592)

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SKU:
CAB1592
Product type:
Antibody
Reactivity:
Human
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
Frequently bought together:

Description

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Antibody Name:Anti-ADD1 Antibody
Antibody SKU:CAB1592
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 60-300 of human ADD1 (NP_054908.2).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse
Positive Samples:BT-474, Mouse brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 60-300 of human ADD1 (NP_054908.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MILQ SPAF CEEL ESMI QEQF KKGK NPTG LLAL QQIA DFMT TNVP NVYP AAPQ GGMA ALNM SLGM VTPV NDLR GSDS IAYD KGEK LLRC KLAA FYRL ADLF GWSQ LIYN HITT RVNS EQEH FLIV PFGL LYSE VTAS SLVK INLQ GDIV DRGS TNLG VNQA GFTL HSAI YAAR PDVK CVVH IHTP AGAA VSAM KCGL LPIS PEAL SLGE VAYH DYHG ILVD EEEK VLIQ KNLG PKSK VLIL R
Gene ID:118
Uniprot:P35611
Cellular Location:Cell membrane, Cytoplasm, Cytoplasmic side, Peripheral membrane protein, cytoskeleton
Calculated MW:56kDa/69kDa/73kDa/80kDa/84kDa
Observed MW:73kDa
Synonyms:ADD1, ADDA, adducin 1
Background:Adducins are a family of cytoskeleton proteins encoded by three genes (alpha, beta, gamma). Adducin is a heterodimeric protein that consists of related subunits, which are produced from distinct genes but share a similar structure. Alpha- and beta-adducin include a protease-resistant N-terminal region and a protease-sensitive, hydrophilic C-terminal region. Alpha- and gamma-adducins are ubiquitously expressed. In contrast, beta-adducin is expressed at high levels in brain and hematopoietic tissues. Adducin binds with high affinity to Ca(2+)/calmodulin and is a substrate for protein kinases A and C. Alternative splicing results in multiple variants encoding distinct isoforms; however, not all variants have been fully described.
UniProt Protein Function:Membrane-cytoskeleton-associated protein that promotes the assembly of the spectrin-actin network. Binds to calmodulin.
NCBI Summary:Adducins are a family of cytoskeletal proteins encoded by three genes (alpha, beta, and gamma). Adducin acts as a heterodimer of the related alpha, beta, or gamma subunits. The protein encoded by this gene represents the alpha subunit. Alpha- and beta-adducin include a protease-resistant N-terminal region and a protease-sensitive, hydrophilic C-terminal region. Adducin binds with high affinity to Ca(2+)/calmodulin and is a substrate for protein kinases A and C. [provided by RefSeq, Aug 2017]
UniProt Code:P35611
NCBI GenInfo Identifier:12644231
NCBI Gene ID:118
NCBI Accession:P35611.2
UniProt Secondary Accession:P35611,Q13734, Q14729, Q16156, Q86XM2, Q9UJB6, A2A3N8 A2A3P0, B4DI79, D3DVR3, D3DVR4, D3DVR5,
UniProt Related Accession:P35611
Molecular Weight:73,333 Da
NCBI Full Name:Alpha-adducin
NCBI Synonym Full Names:adducin 1
NCBI Official Symbol:ADD1  
NCBI Official Synonym Symbols:ADDA  
NCBI Protein Information:alpha-adducin
UniProt Protein Name:Alpha-adducin
UniProt Synonym Protein Names:Erythrocyte adducin subunit alpha
Protein Family:Alpha-adducin
UniProt Gene Name:ADD1  
Anti-ADD1 Antibody (CAB1592)Western blot analysis of extracts of various cell lines, using ADD1 antibody (CAB1592) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

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