ENPEP, also known as aminopeptidase A, is a member of the peptidase M1 family. Members of this family are involved in response to cadmium ion and proteolysis. They located in 6 components and are expressed in 26 plant structures. ENPEP is expressed by epithelial cells of the proximal tubule cells and the glomerulus of the nephron. It also can be detected in a variety of other tissues. ENPEP probably plays a role in regulating growth and differentiation of early B-lineage cells. It also may play a role in the catabolic pathway of the renin-angiotensin system. ENPEP is a zinc-dependent membrane-bound aminopeptidase that catalyzes the cleavage of glutamatic and aspartatic amino acid residues from the N-terminus of polypeptides. It degrades vasoconstricting angiotensin II into angiotensin III and therefore helps to regulate blood pressure.
Product Name:
Mouse ENPEP/Aminopeptidase A Recombinant Protein (RPES4380)
Product Code:
RPES4380
Size:
10µg
Species:
Mouse
Expressed Host:
Baculovirus-Insect Cells
Synonyms:
6030431M22Rik,APA,Bp-1/6C3,ENPEP,Ly-51,Ly51
Accession:
P16406
Sequence:
Arg41-Pro945
Fusion tag:
N-His
Activity:
Measured by its ability to cleave the fluorogenic peptide substrate, Glu-7-amido-4-methyl coumarin.The specific activity is > 200 pmoles/min/µg.
Endotoxin:
<1.0 EU per µg of the protein as determined by the LAL method.
Protein Construction:
A DNA sequence encoding the mature form of mouse ENPEP (P16406) (Arg41-Pro945) was expressed, with a polyhistidine tag at the N-terminus.
Purity:
> 91 % as determined by SDS-PAGE
Mol Mass:
106 kDa
AP Mol Mass:
113 kDa
Formulation:
Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 7.4
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
